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Raman Spectroscopic Signature Of Blood And Its Potential

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applications to various blood samples by many researchers in recent years. Raman spectroscopic signature are combined with chemometric models for blood species discrimination [14–23]. Two major issues addressed in this paper involved the influence of sample heterogeneity and potential Raman spectral variations that could arise between different donors of blood. Advanced statistical analysis of spectra obtained from multiple spots on dry samples showed that dry blood is chemically heterogeneous, and its Raman spectra could be presented very well as a linear Data analysis was taken a step further to better understand the order of the kinetic events, and two-dimensional correlation spectroscopy (2D CoS) of the Raman spectroscopic data was utilized [61]. In addition, all spectra were identified as blood using our previously constructed multidimensional spectroscopic body fluid signatures.

Virkler, K. & Lednev, I. K. Raman spectroscopic signature of blood and its potential application to forensic body fluid identification. Anal Bioanal Chem 396, 525–534, https://doi.org/10.1007 In a study that might someday help solve crimes, researchers used Raman spectroscopy to identify the species of origin from the blood

Raman Spectroscopy of Blood and Blood Components.

Raman Spectroscopic Study of TiO2 Nanoparticles’ Effects on the ...

Abstract This proof-of-concept study demonstrated the potential of Raman microspectroscopy for nondestructive identification of traces of sweat for forensic purposes. Advanced statistical analysis of Raman spectra revealed that dry sweat was intrinsically heterogeneous, and its biochemical composition varies significantly with the donor. The blood spectroscopic signature which contained three major principal components (Figure 1.B), including a fluorescent background,

Virkler K, Lednev IK (2010) Raman spectroscopic signature of blood and its potential application to forensic body fluid identification, Anal Bioanal Chem. 396:525-34. Advantages of Raman spectroscopy include minimal sample preparation, low sample volume requirements, and its non-destructive nature, making it suitable for analyzing solid and liquid samples. This technique has been used to study blood components since the 1970s, with ongoing innovations in instrumentation and applications [24], [25]. Virkler, K.; Lednev, I.K. Raman spectroscopic signature of semen and its potential application to forensic body fluid identification, Forensic Sci. Int. 2009, 193, 56–62.

Fig. 3. The average Raman spectra of five semen samples (black) with the fitted spectroscopic signature (a—e), and the Raman spectra of blood (f) and saliva

Multidimensional spectroscopic signatures consist of several characteristic Raman spectral components representing variations in the biochemical composition of body fluid with donor and spatial heterogeneity of dry traces. Recently, Raman analysis of body fluids, including blood, has been shown to be of potential value for forensic purposes, allowing nondestructive, rapid, on-site identification of human body fluids. 31, 32 Thus the continuing characterization of the Raman activity of human blood and its components may have potential importance

Potential of Raman spectroscopic techniques to study proteins

By combining Raman spectroscopy with multivariate data analysis, we have developed multidimensional spectroscopic signatures for blood, saliva, semen, sweat, and vaginal fluid. (11-15) We have also developed a statistical model to differentiate and identify these body fluids (5) and determined its limit of detection for peripheral The method is based on the use of multidimensional spectroscopic signatures of body fluids and accounts for the intrinsic heterogeneity of dry traces and donor variation. The results pre-sented here demonstrate that Raman spectros-copy has potential for identifying traces of semen, blood, saliva, sweat, and vaginal fluid with high confidence. In this article, we briefly overview our approach for developing a multi-dimensional Raman spectroscopic signature for a single body fluid and discuss our new results on the application of advanced statistical analysis of Raman spectroscopic data for

A great potential of Raman spectroscopy for non-destructive, confirmatory identification of body fluids at the crime scene has been reported recently (Virkler and Lednev, Forensic Sci. Int. 2008). However, that analysis was carried out on only one sample of each body fluid and did not take into Near-infrared (NIR) Raman spectroscopy was used to measure spectra of dried human blood samples from multiple donors. Two major questions addressed in this paper involve the influence of sample heterogeneity and potential Raman spectral variations that could arise between different donors of blood. Advanced statistical analysis of spectra obtained from multiple spots Peripheral blood was taken from ten donors, and prepared for analysis by smearing the blood on microscope slides to isolate individual red blood cells. These cells were analyzed by Raman microspectroscopy, and their spectra were loaded into the previously published classification model [28].

Virkler K, Lednev IK (2010) Raman spectroscopic signature of blood and its potential application to forensic body fluid identification, Anal Bioanal Chem. 396:525-34. Semantic Scholar extracted view of „Raman spectroscopic signature of vaginal fluid and its potential application in forensic body fluid identification.“ by Aliaksandra Sikirzhytskaya et al. As is evident from this figure, and explained in detail in the paper, a multidimensional Raman spectroscopic signature of blood was used to reconstruct a new spectrum, which allowed for successful identification of blood even when the stain was contaminated [41].

(DOI: 10.1117/1.1380668) Raman spectra of whole blood and oxy-hemoglobin (Hb) were measured under the same conditions with visible (514.5 nm) and near-infrared (NIR; 720 and 1064 nm) excitation, and the obtained spectra were compared in detail. The Raman spectrum of blood excited with visible light is dominated by very intense bands due to carotenoids, so that it Request PDF | Multidimensional Raman spectroscopic signature of sweat and its potential application to forensic body fluid identification | This proof-of-concept study demonstrated the potential

Raman spectroscopic probing of blood components and of whole blood has been on-going for more than four decades and has proven useful in applications ranging from the understanding of hemoglobin oxygenation, to the discrimination of cancerous cells from healthy lymphocytes, and the forensic investigation of crime scenes. Article Open access Published: 03 April 2023 A novel Raman spectroscopic method for detecting traces of blood on an interfering substrate Yury V. Kistenev, Alexei V. Borisov, Alisa A. Samarinova

Calculated characteristic Raman and fluorescent spectral components were used to build a multidimensional spectroscopic signature of vaginal fluid, which demonstrated good specificity and was able to handle heterogeneous samples from different donors. Raman spectroscopic signature of semen and its potential application to forensic body fluid identification. Virkler K, Lednev IK Forensic Sci Int, 193 (1-3):56-62, 21 Oct 2009 Cited by: 33 articles | PMID: 19850425 ‪Professor of Chemistry, University at Albany, State University of New York‬ – ‪‪Cited by 18,073‬‬ – ‪forensics‬ – ‪medical diagnostics‬ – ‪protein folding and aggregation‬

Raman spectroscopic signature of vaginal fluid and its potential application in forensic body fluid identification. Sikirzhytskaya A, Sikirzhytski V, Lednev IK Forensic Sci Int, 216 (1-3):44-48, 09 Sep 2011 Cited by: 15 articles | PMID: 21908114 Virkler K, Lednev IK (2009) Raman spectroscopic signature of semen and its potential application to forensic body fluid identification, Forensic Sci Int. doi: 10.1016/j.forsciint.2009.09.005 https://doi.org/10.1016/j.forsciint.2009.09.005 Raman spectroscopic signature of blood and its potential application to forensic body fluid identification. Anal Bioanal Chem. 2010;396:525–34. 10.1007/s00216-009-3207-9 Search in Google Scholar PubMed

Raman spectroscopic signature of blood and its potential application to forensic body fluid identification. Virkler K, Lednev IK Anal Bioanal Chem, 396 (1):525-534, 16 Oct 2009 Cited by: 38 articles | PMID: 19834691 This proof-of-concept study demonstrated the potential of Raman microspectroscopy for nondestructive identification of traces of sweat for forensic purposes. Advanced statistical analysis of Raman spectra revealed that dry sweat was intrinsically heterogeneous, and its biochemical composition varies significantly with the donor. As a result, no single Raman spectrum could

We also investigated the extent to which spectroscopic signatures of blood on undyed cotton could be altered if the body fluid was not dried and if it was removed by washing.