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Next-Generation Pathogen Diagnosis With Crispr/Cas-Based Detection Methods

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Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems and Argonaute (Ago) belong to the Microbial Defense Systems (MDS). The guided, programmable, and targeted activation of nucleases by both of them is leading the way to a new generation of pathogens detection.

CRISPR-Cas-Based Diagnosis of Geminiviruses

CRISPR/Cas-Based Biosensor As a New Age Detection Method for Pathogenic ...

Recent advances in CRISPR-based diagnostics (CRISPR-Dx) have demonstrated the potential for highly specific molecular detection, but the sensitivity is often constrained by the slow trans-cleavage activity of Cas enzymes, necessitating preamplification of target nucleic acids.

Despite the rapid application of next-generation sequencing (NGS) technologies, target sequencing in regions of the genome is often required to diagnose many genetic diseases. Target enrichment can be an effective factor in reducing the cost of sequencing and the duration of sequencing. Recently, several clustered system regularly interspaced short palindromic

Ramachandran V, Weiland JJ, Bolton MD (2021) CRISPR-based isothermal next-generation diagnostic method for virus detection in sugarbeet. Front Microbiol 12:679994 4. Rapid, sensitive, and visual detection of pseudorabies virus with an RPA-CRISPR/EsCas13d-based dual-readout portable platform;Analytica Chimica Acta;2024-08 5. Advances in the applications of CRISPR/Cas system for tumor molecular diagnostics;LabMed Discovery;2024-08 同舟云学术 1.学者识别 学者识别 2.学术分析 学术 Molecular diagnostic (MDx) methods directly detect target nucleic acid sequences and are therefore an important approach for precise diagnosis of pathogen infection. In comparison with traditional MDx techniques such as PCR, the recently developed CRISPR-based diagnostic technologies, which employ

An ideal molecular diagnostic method should be sensitive, specific, low cost, rapid, portable, and easy to operate. Traditional nucleic acid detection methods based mainly on PCR technology have not only high sensitivity and specificity, but also some limitations, such as the need for expensive equipment and skilled technicians, being both time and labor intensive, and

CRISPR-Cas-mediated diagnostics: Trends in Biotechnology

Critical review on where CRISPR meets molecular diagnostics Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas s [Application of CRISPR/Cas systems in the nucleic acid detection of pathogens: a review]. Mining microbial organisms to discover and characterize novel CRISPR-Cas systems

In addition to device integration, CRISPR/Cas-mediated detection will respond with innovations in the Cas enzyme and reagent discovery to realize a one-pot assay with desirable detection limit and reduced assay time. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises. Keyword Nucleic acid detection pathogen diagnostic infection disease detection point-of-care testing CRISPR-Cas Indexed By SCI ; SCIE Language 英语

Overview of the basic mechanism involved while adopting CRISPR as biosensors, starting from (a) sample processing, (b) common reaction pool, (c) Cas effector-based cis–trans cleavage, and (d) post-cleavage detection. Accurate and rapid detection of pathogens is essential for food safety, epidemic prevention, disease diagnosis and environmental monitoring. So far, many CRISPR/Cas systems have been gradually developed as new biosensing tools for biological nucleic acid detection. These effectors are coupled with existing nucleic acid amplification methods to produce

Therefore, advances in the field of virus diagnosis and the development of new point-of-care testing kits become necessary globally. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is an emerging technology for gene editing and diagnostics development. Plant viruses are among the most serious threats to global agriculture, causing significant yield losses and jeopardizing food security. Identifying these viruses is crucial to prevent widespread crop damage and ensure effective management. CRISPR-Cas13a, a subtype of the RNA-targeting Cas13 family, has emerged as a transformative tool in molecular Several CRISPR-based diagnostics have been reported that utilize either the cleavage-based or the binding-based strategy for the detection of nucleic acids (Table 1).

Rapid diagnosis of bloodstream infections is vital for timely effective treatment. Here, Mao and Wan et al developed a method using a dual stem-loop DNA signal amplifier, to achieve ultrasensitive This review explores the various CRISPR-based COVID-19 detection methods and related diagnostic devices. As with any emerging technology, CRISPR/Cas-based nucleic acid testing methods have several challenges that must be overcome for practical applications in clinics and hospitals.

CRISPR-based molecular diagnostics take advantage of the robust and highly specific characteristics of CRISPR/Cas systems to create ultra-sensitive, low-cost, and quick non-laboratory-based detection kits for genetic disorders, viruses, and other pathogens.

Next-Generation Diagnostic with CRISPR/Cas: Beyond Nucleic Acid Detection

CRISPR/Cas-based diagnostics may prove to be a better option to develop ultra-sensitive, inexpensive, and rapid non-laboratory-based detection kits for several viruses and other pathogens. Nowadays, CRISPR diagnostic methods use the trans-cleavage activity of CRISPR-Cas to generate amplified detection signals and incorporate

These methods have a high specificity, versatility, and rapid detection cycle. In this paper, CRISPR-Cas-based detection methods are discussed in depth. Although CRISPR-Cas-mediated pathogen diagnostic solutions face challenges, their powerful capabilities will pave the way for ideal diagnostic tools. This paper reviews the technical principles, advantages, and potential limitations of these existing molecular diagnostic methods, including polymerase chain reaction (PCR) and its derivatives, isothermal amplification, DNA hybridization, genomic sequencing as well as CRISPR/Cas-based detection techniques. On the other hand, rapid antigen based techniques are cost effective and easily accessible, but are less effective in terms of sensitivity and specificity. CRISPR-Cas systems are advanced diagnostic tools that combine the advantages of both PCR and antigen based detection techniques, and allows the rapid detection with high

Amplification-free CRISPR/Cas detection technology: challenges ...

CRISPR-mediated antibody diagnosis technology can recognize thousands of antibodies in a sample simultaneously. The potential of CRISPR-Cas biosensing technologies is inspiring researchers to develop next-generation diagnostics platforms.

Given the rapid development of CRISPR-based diagnostics in recent years, the Cas13a system shows great potential as a next-generation platform for accurate, portable, and cost-effective detection of viral and bacterial diseases.

The advent of CRISPR-Cas genome editing technology has proven its potential for the evolution of next-generation-based diagnostic methods due to its capability of providing high specificity, reliability, and sensitivity [26], but currently, CRISPR-based biosensors for the detection of hepatitis virus are only available for hepatitis The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-related proteins (CRISPR/Cas) are regarded as the next generation of molecular diagnostic systems. Due to its good biocompatibility, unique cutting activity, ease of operation and design, the CRISPR/Cas system plays an important role in biosensing applications, especially

We will then present various nucleic acid and non-nucleic acid detection methods based on CRISPR/Cas systems for diagnosing both infectious and non-infectious diseases, with a particular focus on the detection of single nucleotide polymorphisms (SNPs).

CRISPR Approaches for the Diagnosis of Human Diseases

Visual detection and differentiation of porcine epidemic diarrhea virus wild−type strains and attenuated vaccine strains using CRISPR/Cas13a-based lateral flow strip CRISPR-based diagnostics offer transformative solutions for disease detection, utilizing advanced gene-editing tools for precise and rapid molecular testing.

However, considering that various detection scenarios of pathogen detection, the application of single signal output is relatively limited, and it is expected to develop a universal CRISPR/Cas12a-powered platform for pathogen detection in multi-scenarios with In this review, based on the detection principle, we provide a detailed classification and comprehensive discussion of recent works that harness these CRISPR-based diagnostic tools from a new perspective. Furthermore, current challenges and future perspectives of CRISPR-based diagnostics are outlined. We believe the future of CRISPR/Cas-based diagnostics lies in not only replacing existing methods but also enabling new possibilities, such as detecting rare mutations with 0.1% allele frequency, analyzing unprocessed samples, and identifying

Emerging and relapsing infectious diseases pose a huge health threat to human health and a new challenge to global public health. Rapid, sensitive and simple diagnostic tools are keys to successful management of infectious patients and containment of disease transmission. In recent years, international research on Clustered Regularly Interspaced Short

Bioinformatic pipeline enables high-throughput primer and guide RNA design for sensitive and specific detection of pathogens in CRISPR-based diagnostics.

These findings demonstrate that our RAA-based CRISPR – Cas12a/Cas13a dual-channel detection system can detect two different subtypes of gyrovirus in a sample with good specificity and high sensitivity, improving the detection efficiency and providing a new technique for the study of viral infection dynamics. Introduction The clustered regularly interspaced short palindromic sequence repeats-Cas (CRISPR-Cas) system has emerged as a promising tool for next