Coexpression Of Vhb And Mceg Genes In Mycobacterium Sp.
Di: Ava
Mycobacterium sp. strain SNP11 is able to grow with pyrene, fluoranthene, phenanthrene and fluorene the sole carbon and energy sources. A probe based on the previously described gene pdoA2, which encodes the alpha subunit of a PAH ring-hydroxylating dioxygenase in Mycobacterium sp. strain 6PY1 [S. K In order to analyze the effects of AMY365-1 and VHb overexpression on genes of hypocrellin biosynthesis, relative expression levels of α-amylase gene amy365 – 1, hemoglobin gene vgb, seven hypocrellin biosynthesis genes including fad, mono, zftf, omef, msf, pks, mco and four related genes in central carbon metabolism including pdc, ald, acs
Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation Article Sep 2021 Yang Zhang Xiuling Zhou Yingying Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation Citing article Sep 2021 Yang Zhang Xiuling Zhou Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation Article Sep 2021 BIORESOURCE TECHNOL Yang Zhang
聊城大学药学与食品工程学院
Co-expression of auxiliary genes enhances the activity of a heterologous O 2 -tolerant hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 Sara Lupacchini, Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation. Yang ZhangXiuling Zhou +5 authors Yanbing Shen Environmental Science, Chemistry Bioresource technology 2021 11
BIORESOURCE TECHNOLOGY PMID: 39384046 Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation 点击查看 >> Author:
Results demonstrated that expressions and coexpression of AMY365-1 and VHb not only increased the productions of biomass, amylase, hypocrellin, but also up-regulated relative expression levels of
Request PDF | Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum | Mycobacteria have been modified to transform Furthermore, constitutive expression of Vitreoscilla hemoglobin in Sphingomonas elodea improved gellan gum, an exopolysaccharide (up to 27%) [4]. However, it is still unknown if expression of the VHb gene and the FHb gene can affect biosynthesis of exopolysaccharide in yeast-like fungi and FHb has the similar function to VHb.
He et al. (2018) [32] introduced the MceG of M. tuberculosis into Mycolicibacterium sp. strain MS136-GAB (previously known as Mycobacterium sp. strain MS136) and coexpressed the YrbEA and YrbEB genes, which increased the yield of 9OH-AD by 20%. It was concluded that the Mce4 transporter plays an active role in the uptake and transport of sterols. Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation Yang Zhang, Xiuling Zhou, Yingying Yao, Qianqian Xu,
He et al. (2018) [32] introduced the MceG of M. tuberculosis into Mycolicibacterium sp. strain MS136-GAB (previously known as Mycobacterium Results demonstrated that expressions and coexpression of AMY365-1 and VHb not only increased the productions of biomass, amylase, hypocrellin, but also up-regulated relative expression levels of four central carbon metabolism genes (pdc, ald, acs, acc) and seven hypocrellin biosynthesis genes (fad, mono, zftf, omef, msf, pks, mco). Coexpression of VHb and MceG genes in Mycobacterium sp. strain LZ2 enhances androstenone production via immobilized repeated batch fermentation. Bioresour Technol 342:125965.
Haiying Shi’s research works
He et al. (2018) [32] introduced the MceG of M. tuberculosis into Mycolicibacterium sp. strain MS136-GAB (previously known as Mycobacterium sp. strain MS136) and coexpressed the YrbEA and YrbEB genes, which increased the yield of 9OH-AD by 20%. It was concluded that the Mce4 transporter plays an active role in the uptake and
Results demonstrated that expressions and coexpression of AMY365-1 and VHb not only increased the productions of biomass, amylase, hypocrellin, but also up-regulated relative expression levels of A Vitreoscilla hemoglobin (VHb) gene was efficiently expressed by the optimization of codons and intron addition in G. lucidum. Expression of the VHb gene was confirmed by genome PCR, quantitative real-time PCR and carbon monoxide (CO)-difference spectrum analysis in the transformant. The effects of the efficient expression of VHb gene on production, This involves an active transport process that requires trans-membrane proteins and ATP. A phytosterol transport system from Mycobacterium tuberculosis H37Rv was constructed in Mycobacterium sp. strain MS136 by co-expression of an energy-related gene, mceG, and two integrated membrane protein genes, yrbE4A and yrbE4B.
Streptomyces lydicus A02 is a novel producer of commercially important polyene macrocyclic antibiotic natamycin and a potential biocontrol agent to several plant fungal diseases, including wilt caused by Fusarium oxysporum f. spp. To improve the natamycin production and the antifungal activity of S. lydicus A02, we coexpressed gene vgb encoding Vitreoscilla Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation
Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch
Unraveling the pleiotropic role of the MceG ATPase in Mycobacterium
文献「Mycobacterium sp.LZ2株におけるVHbとMceG遺伝子の共発現は固定化反復バッチ発酵によるアンドロステノン生産を増強する【JST・京大機械翻訳】」の詳細情報です。J-GLOBAL 科学技術総合リンクセンターは、国立研究開発法人科学技術振興機構(JST)が運営する、無料で研究者、文献、特許などの Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation Article Sep 2021 Yang Zhang Xiuling Zhou Yingying Coexpression of VHb and MceG genes in Mycobacterium sp. Strain LZ2 enhances androstenone production via immobilized repeated batch fermentation Article Sep 2021 BIORESOURCE TECHNOL Yang Zhang
Webster (1988) and Bailey (1988) isolated the DNA fragment including the vgb gene, which encodes VHb protein and its native promoter respectively (Dikshit and Webster, 1988, Khosla and Bailey, 1988b). Southern-blot analysis indicated that only a single copy of this gene exists on the chromosome. A phytosterol transport system from Mycobacterium tuberculosis H37Rv was constructed in Mycobacterium sp. strain MS136 by co-expression of an energy-related gene, mceG, and two integrated membrane protein genes, yrbE4A and yrbE4B. Streptomyces lydicus A02 is a novel producer of commercially important polyene macrocyclic antibiotic natamycin and a potential biocontrol agent to several plant fungal diseases, including wilt caused by Fusarium oxysporum f. spp. To improve the natamycin production and the antifungal activity of S. lydicus A02, we coexpressed gene vgb encoding Vitreoscilla
Mycobacteria is important microbial cell factory in the steroid industry, and there is an urgent need to develop synthetic biology toolkit to regulate the expression level of genes in mycobacterial strains. In this study, we first evaluated the adaptation of seven promoters in Mycobacterium neoaurum ATCC 25795, including trp, tac, trc, and tic from Escherichia coli,
Overexpression of the optimized Vitreoscilla hemoglobin (VHb) gene and the native flavohemoglobin (FHb) gene in Aureobasidium melanogenum P16 rendered a V6 strain and a F44 strain to overproduce
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